Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 11, Pages 4304-4312Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr0703728
Keywords
diagonal chromatography; sialic acid; serum; COFRADIC
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A new approach for proteome-wide analysis of sialylated N-glycopeptides based on the diagonal chromatographic COFRADIC technology is presented here. The use of alpha(2-3,6,8,9) neuraminidase is central to isolate sialylated N-glycopeptides out of a complex peptide mixture. Two different COFRADIC techniques are introduced here, either without or with post-metabolic oxygen-18 labeling (direct versus indirect sorting), and when applied to immuno-depleted mouse serum, we herewith identified 93 sialylated glycosylation sites in 53 serum proteins.
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