Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 377, Issue -, Pages 448-455Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2014.05.012
Keywords
MALDI; IR-MALDESI; Mass spectrometry imaging; Drug distribution
Funding
- National Institutes of Health [R01GM087964]
- GlaxoSmithKline
- William R. Kenan, Jr. Fund for Engineering, Technology Science
- W. M. Keck Foundation
- North Carolina State University
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Determining the distribution of a drug and its metabolites within tissue is a key facet of evaluating drug candidates. Drug distribution can have a significant implication in appraising drug efficacy and potential toxicity. The specificity and sensitivity of mass spectrometry imaging (MSI) make it a perfect complement to the analysis of drug distributions in tissue. The detection of lapatinib as well as several of its metabolites in liver tissue was determined by MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) coupled to high resolving power Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers. IR-MALDESI required minimal sample preparation while maintaining high sensitivity. The effect of the electrospray solvent composition on IR-MALDESI MSI signal from tissue analysis was investigated and an empirical comparison of IR-MALDESI and UV-MALDI for MSI analysis is also presented. (C) 2014 Elsevier B.V. All rights reserved.
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