Journal
JOURNAL OF GENERAL VIROLOGY
Volume 88, Issue -, Pages 2935-2940Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/vir.0.83019-0
Keywords
-
Categories
Funding
- MRC [G9202171, G9800943] Funding Source: UKRI
- Medical Research Council [G9800943, G9202171] Funding Source: Medline
- Medical Research Council [G9202171, G9800943] Funding Source: researchfish
Ask authors/readers for more resources
The cellular protein human Daxx (hDaxx), a component of nuclear domain 10 structures, is known to mediate transcriptional repression of human cytomegalovirus immediate-early (IE) gene expression upon infection of permissive cell types, at least in part, by regulation of chromatin structure around the major IE promoter (MIEP). As it is now clear that differentiation-dependent regulation of the MIEP also plays a pivotal role in the control of latency and reactivation, we asked whether hDaxx-mediated repression is involved in differentiation-dependent MIEP regulation. We show that downregulation of hDaxx by using small interfering RNA technology in undifferentiated NT2D1 cells does not permit expression of viral IE genes, nor does it result in changes in chromatin structure around the MIEP. Viral IE gene expression is only observed upon cellular differentiation, suggesting little involvement of hDaxx in the regulation of the viral MIEP in undifferentiated cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available