Journal
EMBO REPORTS
Volume 8, Issue 11, Pages 1080-1085Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.embor.7401082
Keywords
Tel1; Mec1; Tbf1; STEX; telomerase; telomere length homoeostasis
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Telomerase enables telomere length homeostasis, exhibiting increasing preference for telomeres as their lengths decline. This regulation involves telomere repeat- bound Rap1, which provides a length- dependent negative feedback mechanism, and the Tel1 and Mec1 kinases, which are positive regulators of telomere length. By analysing telomere elongation of wild- type chromosome ends at single- molecule resolution, we show that in tel1 Delta cells the overall frequency of elongation decreases considerably, explaining their short telomere phenotype. At an artificial telomere lacking a subtelomeric region, telomere elongation no longer increases with telomere shortening in tel1 Delta cells. By contrast, a natural telomere, containing subtelomeric sequence, retains a preference for the elongation of short telomeres. Tethering of the subtelomere binding protein Tbf1 to the artificial telomere in tel1 Delta cells restored preferential telomerase action at short telomeres; thus, Tbf1 might function in parallel to Tel1, which has a crucial role in a TG- repeat- controlled pathway for the activation of telomerase at short telomeres.
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