Pseudidiomarina sediminum sp nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China

A strain of heterotrophic, facultatively anaerobic, marine bacterium, designated strain c121(T), was isolated from coastal sediment of Luoyuan Bay, in Fujian province, PR China. Analysis of the 16S rRNA gene sequence revealed an affiliation with the genus Pseudidiomarina; the sequence similarity between c121(T) and Pseudidiomarina taiwanensis PIT1(T) was 97%. Cells of the novel strain were non-pigmented, Gram-negative rods, 0.3 mu m wide and 1.2-1.8 mu m long. Cells grown in broth cultures were non-motile, lacking flagella. Growth of the strain was observed at salinities ranging from 0.5 to 15% NaCl, and the optimal concentration was about 1-8%. The temperature range for growth was rather broad and was high for a marine bacterium: the strain grew at 13-42 degrees C, showed good growth at 20-40 degrees C and had an optimum between 30 and 40 degrees C. The major cellular fatty acids were iso-C-15:0 (24.2%), C-16:1 omega 7c/iso-C-15:0 2-OH (15.3%) and iso-C-17:1 omega 9c (11.9%). The DNA G + C content was 50.0 mol%. Phylogeny based on 16S rRNA gene sequences, together with data from phenotypic and chemotaxonomic characterization, revealed that strain c121(T) could be classified within a novel species of the genus Pseudidiomarina, for which the name Pseudidiomarina sediminum sp. nov. is proposed, with the type strain c121(T) (=CICC 10319(T) =LMG 24046(T)).