Journal
CELL AND TISSUE RESEARCH
Volume 330, Issue 2, Pages 221-229Publisher
SPRINGER
DOI: 10.1007/s00441-007-0455-x
Keywords
peritoneal mesothelial cells; dipeptidyl peptidase IV; transforming growth factor-beta 1; migration; stromal-cell-derived factor-1 alpha; wound healing; human
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We have previously reported that human peritoneal mesothelial cells (HPMCs) express a large amount of dipeptidyl peptidase IV (DPPIV) and that its expression is regulated by a variety of bioactive substances in malignant ascites from ovarian cancer patients. The aim of this study has been to examine the expression and role of the SDF-1 alpha/CXCR4-DPPIV axis in HPMCs. We have demonstrated that the expression levels of DPPIV and E-cadherin in HPMCs decrease, following TGF-beta 1-induced morphological change, in a time- and concentration-dependent manner. Additionally, we show that both SDF-1 alpha (a chemokine and substrate for DPPIV) and its receptor, CXCR4, are expressed on HPMCs, and that their expression levels are upregulated by TGF-beta 1 treatment, resulting in an increased migratory potential of HPMCs. Furthermore, the migratory potential of HPMCs is significantly enhanced in the presence of SDF-1 alpha or DPPIV-specific inhibitor in the wound-healing assay. These results suggest that DPPIV and SDF-1 alpha/CXCR4 play crucial roles in regulating the migratory potential of HPMCs, which may be involved in the re-epithelialization of denuded basement membrane at the site of peritoneal injury.
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