Journal
FERTILITY AND STERILITY
Volume 88, Issue 5, Pages 1318-1326Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2007.01.038
Keywords
AZFc partial deletion; male infertility; spermatogenic impairment; Y chromosome; DAZ gene
Categories
Ask authors/readers for more resources
Objective: To determine the prevalence of AZFc subdeletions in infertile Chilean men with severe spermatogenic impairment. Design: Prospective analysis. Setting: University infertility clinic. Patient(s): Ninety-five secretory azo/oligozoospermic men without AZFc Y chromosome microdeletions: 71 whose testicular histology showed severe spermatogenic impairment and 24 who exhibited reduced testicular volume and elevated serum FSH levels. As controls, we studied 77 men (50 fertile and/or normozoospermic, and 27 with azoospermia and normal spermatogenesis). Intervention(s): Peripheral blood was drawn to obtain genomic DNA for polymerase chain reaction (PCR) digestion assays of DAZ-sequence nucleotide variants and for AZFc-STS PCR after a complete testicular characterization (biopsy, hormonal, and physical evaluation). Main Outcome Measure(s): DAZ genes and AZFc subdeletion types. Result(s): In cases we observed two gr/gr subdeletions (2.1%), one with absence of DAZ1/DAZ2 (g1/g2 subtype), and the other with absence of DAZ3/DAZ4 (r2/r4 subtype). Additionally, we found a g1/g3 subdeletion in a patient with Sertoli-cell-only syndrome. In controls, we observed two gr/gr subdeletions with absence of DAZ1/DAZ2 (2.6%) in a fertile/normozoospermic and in an obstructive azoospermic man. Conclusion(s): AZFc subdeletions do not seem to cause severe impairment of spermatogenesis. Moreover, gr/gr-DAZ1/DAZ2 subdeletions do not appear to affect fertility in Chilean men.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available