Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 71, Issue 11, Pages 2712-2719Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70306
Keywords
allspice; histone acetyltransferase; androgen receptor; acetylation; prostate cancer
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Funding
- Rural Development Administration (RDA), Republic of Korea [20070301034007] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 mu g/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 mu g/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment. Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 mu g/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.
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