Journal
AMERICAN JOURNAL OF RHINOLOGY
Volume 21, Issue 6, Pages 658-663Publisher
OCEAN SIDE PUBLICATIONS INC
DOI: 10.2500/ajr.2007.21.3094
Keywords
acrolein; defensin; epithelial; IL-8; immunity; innate; nasal; rhinosinusitis; tobacco
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Background: Tobacco use is associated with poorer outcomes of medical and surgical therapy for chronic rhinosinusitis (CRS), although the underlying mechanism is unknown. Acrolein (AC) is a major component of cigarette smoke that has been shown to suppress innate immune gene expression by human bronchial epithelial cells and murine macrophages. In this study, we explore whether exposure of human sinonasal epithelial cells (HSNECs) to AC similarly reduces their innate immune gene expression. Methods: Primary HSNECs from CRS patients were grown in culture, either differentiated or submerged. HSNECs were treated for 30 minutes with 0-50 mu M of AC and were subsequently analyzed by real-time polymerase chain reaction and ELISA to determine IL-8 and human beta-defensin (HBD) 2 expression. Total glutathione was measured to see the oxidative stress within the treatment range. Results: In primary HSNEC, IL-8 mRNA levels decreased dose dependently in the range of 10-50 [mu M of AC with an eightfold decrease at 50 mu M. In addition, a 125-fold decrease at 50 p M for IL-8 protein was observed. HBD-2 mRNA decreased twofold and HBD-2 protein decreased fourfold at 50 mu M of AC in primary HSNEC. However, differentiated HSNEC showed a marginal decrease in a dose-dependent manner for both IL-8 and HBD-2 within the range of 10-50 mu M of AC. There was no oxidative stress observed over this range of AC concentration. Conclusion: The tobacco smoke component AC has the capacity to suppress the inflammatory and innate immune function of sinonasal epithelial cells. Whether this effect contributes to the negative clinical impact ( smoking on CRS outcomes merits additional investigation.
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