Journal
PLANT PHYSIOLOGY
Volume 145, Issue 3, Pages 831-842Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.107.102236
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Funding
- National Research Foundation of Korea [2007-06232] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [20070401034001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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P-1B-type heavy-metal ATPases (HMAs) are transmembrane metal- transporting proteins that play a key role in metal homeostasis. Despite their importance, very little is known about their functions in monocot species. We report the characterization of rice (Oryza sativa) OsHMA9, a member of the P (1B)- type ATPase family. Semiquantitative reverse transcription- polymerase chain reaction analyses of seedlings showed that OsHMA9 expression was induced by a high concentration of copper (Cu), zinc (Zn), and cadmium. We also determined, through promoter:: beta- glucuronidase analysis, that the main expression was in the vascular bundles and anthers. The OsHMA9: green fluorescence protein fusion was localized to the plasma membrane. Heterologous expression of OsHMA9 partially rescued the Cu sensitivity of the Escherichia coli copA mutant, which is defective in Cu- transporting ATPases. It did not rescue the Zn sensitivity of the zntA mutant, which is defective in Zn- transporting ATPase. To further elucidate the functional roles of OsHMA9, we isolated two independent null alleles, oshma9- 1 and oshma9-2, from the T- DNA insertion population. Mutant plants exhibited the phenotype of increased sensitivity to elevated levels of Cu, Zn, and lead. These results support a role for OsHMA9 in Cu, Zn, and lead efflux from the cells. This article is the first report on the functional characterization of a P-1B- type metal efflux transporter in monocots.
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