Journal
JOURNAL OF SEPARATION SCIENCE
Volume 30, Issue 17, Pages 3011-3017Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200700329
Keywords
cation exchange; monolithic column; opium alkaloids; pericarpium papaveris; pressurized capillary electrochromatography
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A method for the separation and determination of five major opium alkaloids (narcotine, papaverine, thebaine, codeine, and morphine) in pericarpium papaveris by pressurized CEC (pCEC) with monolithic column has been developed. Under the optimum condition, linear calibration ranges of narcotine, papaverine, thebaine, codeine, and morphine were obtained as 2-85, 2-85, 5-75, 10-65, and 1065 mu g/mL, respectively. LODs of these analytes were 1.5-6.0 mu g/mL. The RSD (n = 7) of the migration time and peak area were 1.94-5.24 and 4.05-8.21%, respectively. The proposed method was successfully applied to the analysis of pericarpium papaveris samples. Average recoveries of 79.0-95.9% at different fortified levels of alkaloids were achieved with RSD less than 4.6%. Meanwhile, the mechanism of the separation of the alkaloids on the monolithic column was also discussed. The result showed that the separation of alkaloids was mainly based on the mixed mode of hydrophilic interaction (HI) and cation exchange.
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