Journal
PEPTIDES
Volume 28, Issue 11, Pages 2199-2210Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2007.08.011
Keywords
angiotensin-(1-7); NMR studies and ROESY; cyclodextrins; inclusion compounds; QM/MM; DFTB and UFF simulation
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We report the complete sequence-specific hydrogen NMR assignments of vasoactive peptide angiotensin-(1-7) (Ang-(1-7)). Assignments of the majority of the resonances were accomplished by COSY, TOCSY, and ROESY peak coordinates at 400 MHz and 600 MHz. Long-side-chain amino acid spin system identification was facilitated by long-range coherence transfer experiments (TOCSY). Problems with overlapped resonance signals were solved by analysis of heteronuclear 2D experiments (HSQC and HMBC). Nuclear Overhauser effects (NOE) results were used to probe peptide conformation. We show that the inclusion of the angiotensin-(1-7) tyrosine residue is favored in inclusion complexes with (3-cyclodextrin. QM/MM simulations at the DFTB/UFF level confirm the experimental NMR findings and provide detailed structural information on these compounds in aqueous solution. (C) 2007 Elsevier Inc. All rights reserved.
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