Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 27, Issue 21, Pages 7497-7510Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00687-07
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Funding
- NEI NIH HHS [EY11910, R01 EY011910-06S1, R01 EY011910, R01 EY011910-08, R01 EY011910-06, R01 EY011910-07] Funding Source: Medline
- NIDCR NIH HHS [R01 DE012728, DE-12728] Funding Source: Medline
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Activating protein 2 alpha (AP-2 alpha) is known to be expressed in the retina, and AP-2 alpha-null mice exhibit defects in the developing optic cup, including patterning of the neural retina (NR) and a replacement of the dorsal retinal pigmented epithelium (RPE) with NR. In this study, we analyzed the temporal and spatial retinal expression patterns of AP-2 alpha and created a conditional deletion of AP-2 alpha in the developing retina. AP-2 alpha exhibited a distinct expression pattern in the developing inner nuclear layer of the retina, and colocalization studies indicated that A.P-2 alpha was exclusively expressed in postmitotic amacrine cell populations. Targeted deletion of AP-2 alpha in the developing retina did not result in observable retinal defects. Further examination of AP-2 alpha-null mutants revealed that the severity of the RPE defect was variable and, although defects in retinal lamination occur at later embryonic stages, earlier stages showed normal lamination and expression of markers for amacrine and ganglion cells. Together, these data demonstrate that, whereas AP-2 alpha alone does not play an intrinsic role in retinogenesis, it has non-cell-autonomous effects on optic cup development. Additional expression analyses showed that multiple A-P-2 proteins are present in the developing retina, which will be important to future studies.
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