Journal
JOURNAL OF MICROSCOPY
Volume 228, Issue 2, Pages 165-173Publisher
WILEY
DOI: 10.1111/j.1365-2818.2007.01860.x
Keywords
activity; development; CLSM; mitochondrial mass; sea urchin
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In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.
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