Journal
NATURE METHODS
Volume 4, Issue 11, Pages 943-950Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1105
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We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two- photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two- photon calcium imaging in an all-optical method to image and manipulate circuit activity.
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