Journal
PLANT BIOLOGY
Volume 9, Issue 6, Pages 776-785Publisher
BLACKWELL PUBLISHING
DOI: 10.1055/s-2007-965258
Keywords
nitrogen-fixing root nodules; leghemoglobin; hemoglobin; nitric oxide; glb3; trHb
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Three types of hemoglobins exist in higher plants, symbiotic, non-symbiotic, and truncated hemoglobins. Symbiotic (class 11) hemoglobins play a role in oxygen supply to intracellular nitrogen-fixing symbionts in legume root nodules, and in one case (Parasponia sp.), a non-symbiotic (class 1) hemoglobin has been recruited for this function. Here we report the induction of a host gene, dgtrHb1, encoding a truncated hemoglobin in Frankia-induced nodules of the actinorhizal plant Datisca glomerata. Induction takes place specifically in cells infected by the microsymbiont, prior to the onset of bacterial nitrogen fixation. A bacterial gene (Frankia trHbO) encoding a truncated hemoglobin with O-2-binding kinetics suitable for the facilitation Of 02 diffusion (Tjepkema et al., 2002) is also expressed in symbiosis. Nodule oximetry confirms the presence of a molecule that binds oxygen reversibly in D. glomerata nodules, but indicates a low overall hemoglobin concentration suggesting a local function. Frankia TrHbO is likely to be responsible for this activity. The function of the D. glomerata truncated hemoglobin is unknown; a possible role in nitric oxide detoxification is suggested.
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