Journal
NATURE CHEMICAL BIOLOGY
Volume 3, Issue 11, Pages 707-708Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.2007.31
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Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (< 2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells.
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