Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 45, Issue 11, Pages 3802-3803Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01127-07
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We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.
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