Journal
JOURNAL OF IMMUNOLOGY
Volume 179, Issue 9, Pages 6064-6071Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.179.9.6064
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Funding
- NIAID NIH HHS [AI632026, AI065639, AI23283] Funding Source: Medline
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Ab class switching occurs by an intrachromosomal recombination and requires generation of double-strand breaks (DSBs) in Ig switch (S) regions. Activation-induced cytidine deaminase (AID) converts cytosines in S regions to uracils, which are excised by uracil DNA glycosylase (UNG). Repair of the resulting abasic sites would yield single-strand breaks (SSBs), but how these SSBs are converted to DSBs is unclear. In mouse splenic B cells, we find that AID-dependent DSBs occur in S mu mainly in the G, phase of the cell cycle, indicating they are not created by replication across SSBs. Also, G, phase cells express AID, UNG, and mismatch repair (MMR) proteins and possess UNG activity. We find fewer S region DSBs in XIMR-deficient B cells than in wild-type B cells, and still fewer in MMR-deficient/S mu TR-/- B cells, where targets for AID are sparse. These DSBs occur predominantly at AID targets. We also show that nucleotide excision repair does not contribute to class switching. Our data support the hypothesis that MMR is required to convert SSBs into DSBs when SSBs on opposite strands are too distal to form DSBs spontaneously.
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