Glycosylation of tocopherols by cultured cells of Eucalyptus perriniana

Cultured suspension cells of Eucalyptus perriniana converted exogenously administered alpha-tocopherol into alpha-tocopheryl 6-O-beta-D-glucopyranoside (46 mu g/g fr. wt of cells) and two biotransformation products: alpha-tocopheryl 6-O-(6-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside (19 mu g/g fr. wt of cells) and alpha-tocopheryl 6-O-(6-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside (6 mu g/g fr. wt of cells). On the other hand, two other compounds, i.e., delta-tocopheryl 6-O-(6-O-beta-D-glucopyranosyl)-beta-D-glucopyranoside (27 mu g/g fr. wt of cells) and delta-tocopheryl 6-O-(6-O-alpha-L-rhamnopyran osyl)-beta-D-glucopyrano side (12 mu g/g fr. wt of cells), together with delta-tocopheryl 6-O-beta-D-glucopyranoside (63 mu g/g fr. wt of cells) were isolated from suspension cells following the administration of delta-tocopherol. (C) 2007 Elsevier Ltd. All rights reserved.