Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 12, Issue 6, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2818045
Keywords
deep imaging; reflection-mode imaging; photoacoustic (PA) imaging; brain cortex; spleen
Funding
- NIBIB NIH HHS [R01 EB000712] Funding Source: Medline
- NINDS NIH HHS [R01 NS46214, R01 NS046214] Funding Source: Medline
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A reflection-mode photoacoustic (PA) imaging system was designed and built to image deep structures in biological tissues. We chose near-infrared laser pulses of 804-nm wavelength for PA excitation to achieve deep penetration. To minimize unwanted surface signals, we adopted dark-field ring-shaped illumination. This imaging system employing a 5-MHz spherically focused ultrasonic transducer provides penetration up to 38 mm in chicken breast tissue. At the 19-mm depth, the axial resolution is 144 mu m and the transverse resolution is 560 mu m. Internal organs of small animals were imaged clearly. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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