Journal
PROTEIN ENGINEERING DESIGN & SELECTION
Volume 20, Issue 11, Pages 525-534Publisher
OXFORD UNIV PRESS
DOI: 10.1093/protein/gzm046
Keywords
DsRed; fast maturation; fluorescent protein; monomeric; structure
Funding
- NCRR NIH HHS [RR07 707] Funding Source: Medline
- NIBIB NIH HHS [1 R01 EB01 566] Funding Source: Medline
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The red fluorescent protein DsRed has been extensively engineered for use as an in vivo research tool. In fast maturing DsRed variants, the chromophore maturation half-time is similar to 40 min, compared to similar to 12 h for wild-type DsRed. Further, DsRed has been converted from a tetramer into a monomer, a task that entailed mutating approximately 20% of the amino acids. These engineered variants of DsRed have proven extremely valuable for biomedical research, but the structural basis for the improved characteristics has not been thoroughly investigated. Here we present a 1.7 angstrom crystal structure of the fast maturing tetrameric variant DsRed.T4. We also present a biochemical characterization and 1.6 angstrom crystal structure of the monomeric variant DsRed.M1, also known as DsRed-Monomer. Analysis of the crystal structures suggests that rearrangements of Ser69 and Glu215 contribute to fast maturation, and that positioning of the Lys70 side chain modulates fluorescence quantum yield. Despite the 45 mutations in DsRed.M1 relative to wild-type DsRed, there is a root-mean-square deviation of only 0.3 angstrom between the two structures. We propose that novel intramolecular interactions in DsRed.M1 partially compensate for the loss of intermolecular interactions found in the tetramer.
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