Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 22, Pages 7471-7473Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00978-07
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/B/08868] Funding Source: researchfish
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Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.
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