Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 11, Pages 1793-1796Publisher
SPRINGER
DOI: 10.1007/s10529-007-9467-6
Keywords
binary vector; GFP; GUSPlus; plant transformation; segregation analysis
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A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.
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