Journal
MACROMOLECULAR BIOSCIENCE
Volume 7, Issue 11, Pages 1181-1186Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200700162
Keywords
activation energy; biopolymers; differential scanning calorimetry (DSC); kinetics (polym.); proteins
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An isoconversional method is proposed to be used for evaluating activation energy of protein denaturation. Applied to DSC data on collagen denaturation, the method yields an activation energy that decreases throughout the process. The Lumry-Eyring model gives an explanation for this decrease and affords estimates for the enthalpy of the reversible step and the activation energy of the irreversible step of denaturation. The reversible unfolding is detectable by multi-frequency temperature-modulated DSC.
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