Journal
FEBS LETTERS
Volume 581, Issue 27, Pages 5247-5254Publisher
WILEY
DOI: 10.1016/j.febslet.2007.10.012
Keywords
ES cells; Wnt signaling; stemness; beta-catenin
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ES cells can self-renew while preserving pluripotency and are able to differentiate into many cell types. In both processes, different signal transduction pathways are implicated, including the Wnt/beta-catenin pathway, which we here further analyzed. We found that a loss of beta-catenin in ES cells leads to altered expression of stem cell marker genes. TCF/beta-catenin reporter gene assays indicate that undifferentiated murine ES cells are capable of reacting to LiCl and Wnt3a but not Wnt5a stimulation, but have low endogenous TCF/beta-catenin activity. Oct-3/4, nanog and Wntl1 were able to repress TCF/beta-catenin transcriptional activity. During differentiation, activation of the Wnt/beta-catenin pathway influences formation of mesoderm and cardiomyocytes in a time and dose dependent manner. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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