Shrub encroachment into mesic mountain grasslands in the Iberian peninsula:: Effects of plant quality and temperature on soil C and N stocks

This paper aims at understanding the effect that shrub encroachment into mesic mountain grasslands has on soil organic carbon ( SOC) stocks. Thus we compared organic C and N contents in contiguous soil profiles under a conifer shrub, a legume shrub, and grasses ( mesic grasslands) on 21 sites. Soil C and N recalcitrance indexes ( RIC and RIN) were estimated as the ratio of unhydrolyzable C and N to total C or N. Contrary to our hypothesis, shrub encroachment did not cause a well-defined change in soil C stocks. Only a slight increase in C was detected in the top 15 cm soil layer under both shrubs. The C accretion rate in this soil layer was estimated to be approximately 28- 42 g m(-2) per annum. Legume shrub encroachment also produced a slight decrease in the C/N ratio in the top 15 cm soil layer. No significant changes in the RIC were detected as a result of encroachment; however, slight decreases in the RIN were found at medium depths. Both RIC and RIN showed a negative relationship with site temperature in the upper legume-shrub soil layers but not in conifer shrubs or under grasses, suggesting a priming effect on the recalcitrant soil stocks produced by the higher-quality inputs derived from legume encroachment.