Serum calprotectin concentrations in dogs with idiopathic inflammatory bowel disease

Objective-To measure serum calprotectin concentration in dogs with inflammatory bowel disease (IBD) before and after initiation of treatment and evaluate its correlation with a clinical scoring system (canine IBD activity index), serum canine C-reactive protein concentration, and severity of histopathologic changes. Animals-34 dogs with idiopathic IBD and 139 healthy control dogs. Procedures-From dogs with IBD, blood samples were collected immediately before (baseline) and 3 weeks after initiation of 1 of 2 treatments: prednisone (1 mg/kg, PO, q 12 h; n = 211 or a combination of prednisone and metronidazole (10 mg/kg, PO, q 12 h; 13). Blood samples were collected once from each of the control dogs. For all samples, serum calprotectin concentration was determined via radioimmunoassay. Results-Mean serum calprotectin concentrations for dogs with IBD at baseline (431.1 mu g/L) and 3 weeks after initiation of treatment (676.9 mu g/L) were significantly higher, compared with that (219.4 mu g/L) for control dogs, and were not significantly correlated with the canine IBD activity index, serum C-reactive protein concentration, or severity of histopathologic changes. The use of a serum calprotectin concentration of >= 296.0 mu g/L as a cutoff had a sensitivity of 82.4% (95% confidence interval, 65.5% to 93.2%) and specificity of 68.4% (95% confidence interval, 59.9% to 76.0%) for distinguishing dogs with idiopathic IBD from healthy dogs. Conclusions and Clinical Relevance-Serum calprotectin concentration may be a useful biomarker for the detection of inflammation in dogs, but the use of certain drugs leg, glucocorticoids) appears to limit its clinical usefulness. (Am J Vet Res 2012;73:1900-1907)