Journal
FEBS LETTERS
Volume 581, Issue 28, Pages 5382-5388Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.10.037
Keywords
apoptosis; protease; nucleosome; nucleosome releasing factor
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We observed that interaction of secondary necrotic (sn) cells with human serum or plasma leads to loss of DNA staining. The decrease turned out to be a result of nucleosome release and was specific for apoptotic cells as necrotic cells did not show this phenomenon. We named this activity in plasma nucleosome releasing factor (NRF). NRF activity was completely inhibited by trypsin inhibitors suggesting that a serine protease is involved. Upon testing a number of plasma candidate serine proteases we found that plasmin did have NRF activity. However, plasminogen-deficient plasma still had NRF activity indicating that NRF is not plasmin. We conclude that a yet unidentified plasma serine protease is involved in removal of nucleosomes from sn cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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