Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 48, Pages 18886-18891Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0610868104
Keywords
airway reopening; small airway epithelial cells; mechanical forces; microfluidic cell culture
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Funding
- NHLBI NIH HHS [R01 HL084370, HL084370-01] Funding Source: Medline
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We describe a microfabricated airway system integrated with computerized air-liquid two-phase microfluiclics that enables on-chip engineering of human airway epithelia and precise reproduction of physiologic or pathologic liquid plug flows found in the respiratory system. Using this device, we demonstrate cellular-level lung injury under flow conditions that cause symptoms characteristic of a wide range of pulmonary diseases. Specifically, propagation and rupture of liquid plugs that simulate surfactant-deficient reopening of closed airways lead to significant injury of small airway epithelial cells by generating deleterious fluid mechanical stresses. We also show that the explosive pressure waves produced by plug rupture enable detection of the mechanical cellular injury as crackling sounds.
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