Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 55, Issue 24, Pages 9764-9768Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf0715875
Keywords
moniliformin; Fusarium; hydrophilic interaction chromatography; zwitterionic detection; HPLC; maize
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A novel HPLC method was developed for detection of the Fusarium mycotoxin, moniliformin in whole maize plants. The method is based on hydrophilic interaction chromatography (HILIC) on a ZIC zwitterion column combined with diode array detection and negative electrospray mass spectrometry (ESI--MS). Samples were extracted using acetonitrile-water (85:15), and the extracts were cleaned up on strong anion exchange columns. By this procedure we obtained a recovery rate of 57-74% moniliformin with a limit of detection at 48 ng/g and a limit of quantification at 96 ng/g using UV detection at 229 nm, which is comparable to current methods used. Limit of detection and quantification using ESI--MS detection was 1 and 12 ng/g, respectively. Screening of maize samples infected with the moniliformin producing fungi F. avenaceum, F tricinctum, or F subglutinans detected moniliformin levels of 1-12 ng/g in 15 of 28 samples using ESI--MS detection, To our knowledge this is the first example of HILIC separation in mycotoxin analysis.
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