Journal
BIOSENSORS & BIOELECTRONICS
Volume 23, Issue 4, Pages 556-561Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.06.012
Keywords
microperoxidase-11; mixed self-assembly; H2O2; uricase; uric acid biosensor
Ask authors/readers for more resources
Electrochemistry of microperoxidase-11 (MPx-11) anchored on the rnixed self-assembled monolayer (SAM) of 2-(2-mercaptoethylpyrazine) (PET) and 4,4'-dithiodibutyric acid (DTB) on gold (Au) electrode and the biosensing of uric acid (UA) is described. MPx-11 has been covalently anchored on the mixed SAM of PET and DTB on Au electrode. MPx-11 on the mixed self-assembly exhibits reversible redox response characteristic of a surface confined species. The heterocyclic ring of PET promotes the electron transfer between the electrode and the redox protein. The apparent standard rate constant k(s)(app) obtained for the redox reaction of MPx-11 on the mixed monolayer is similar to 2.15 times higher than that on the single monolayer of DTB modified electrode. MPx-11 efficiently mediates the electrocatalytic reduction of H2O2. MPx-11 electrode is highly sensitive to H2O2 and it shows linear response for a wide concentration range. The electrocatalytic activity of the MPx-11 electrode is combined with the enzymatic activity of uricase (UOx) to fabricate uric acid biosensor. The bienzyme assembly is highly sensitive towards UA and it could detect UA as low as 2 mu M at the potential of -0.1 V. The biosensor shows linear response with a sensitivity of 3.4 +/- 0.08 nA cm(-2) mu M-1. Ascorbate (AA) and paracetamol (PA) do not significantly interfere in the amperometric sensing of UA. (c) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available