Journal
GENOMICS
Volume 90, Issue 6, Pages 712-722Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2007.08.009
Keywords
tick; Anaplasma marginale; RNA interference; IDE8 cells
Funding
- Wellcome Trust Funding Source: Medline
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The coevolution of ticks and the pathogens that they transmit has ensured their Mutual survival. In these studies, we used a functional genomics approach to characterize tick genes regulated in response to Anaplasma marginale, infection. Differentially regulated genes/proteins were identified by suppression-subtractive hybridization and differential in-gel electrophoresis analyses of cultured IDES tick cells infected with A. 121(marginale. Nine of 17 of these genes were confirmed by real-time RT-PCR to be differentially regulated in ticks and/or IDES tick cells in response to A marginale infection. RNA interference was used for functional studies. Six genes, which encode putative selenoprotein W2a, hematopoietic stem/progenitor cells protein-like, proteasome 26S Subunit, ferritin, GST, and subolesin control, were found to affect A. marginale infection in IDES tick cells. Four genes, which encode putative GST, salivary selenoprotein M, vATPase, and ubiquitin, affected A. marginale infection in different sites of development in ticks. The results of these studies demonstrated that a molecular mechanism occurs by which tick cell gene expression mediates the A. marginale developmental cycle and trafficking through ticks. (c) 2007 Elsevier Inc. All rights reserved.
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