Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 24, Pages 7997-8000Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00956-07
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Funding
- NIAAA NIH HHS [R25 AA021304, R03 AA020101] Funding Source: Medline
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A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.
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