Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 128, Issue 6, Pages 926-935Publisher
OXFORD UNIV PRESS INC
DOI: 10.1309/JN3NTHK4VVWKJT4A
Keywords
Francisella tularensis; subspecies identification; tularemia; pulsed-field gel electrophoresis; amplified fragment length polymorphism; ribotyping; multilocus variable number tandem repeat analysis; raman spectroscopy
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Rapid methods are needed for public health and military applications to specifically identify Francisella tularensis, the causative agent of tularemia in humans. A comparative analysis of the capabilities of multiple technologies was performed using a well-defined set of organisms to determine which approach would provide the most information in the shortest time. High-resolution molecular techniques, including pulsed-field gel electrophoresis, amplified fragment length polymorphism, and ribotyping, provided subspecies level identification within approximately 24 hours after obtaining an isolate, whereas multilocus variable number tandem repeat analysis with 8 or 25 targets provided strain level discrimination within about 12 hours. In contrast, Raman spectroscopy provided species level identification in 10 minutes but could not differentiate between subspecies tularensis and holarctica.
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