Free radical scavenging activity of gossypin and nevadensin:: An in-vitro evaluation

Objectives: The antioxidant potential of gossypin and nevadensin, two flavonoid compounds, were evaluated by in vitro methods. Materials and Methods: Gossypin, nevadensin, and the reference standard, butylated hydroxyl toluene (BHT), were evaluated for DPPH (1,1-diphenyl-2-picrylhydrazyl), nitric oxide, superoxide, and hydroxyl radical scavenging activity. Results: Gossypin and BHT showed the potential for significant DPPH radical inhibition of up to 88.52 and 91.45% at 100 mu g/ml concentration. With a 100 mu g/ml concentration of gossypin, the in vitro nitric oxide, superoxide, and hydroxyl radical scavenging activity was found to be 74.00, 74.22, and 67.15%, respectively; and with 100 mu g/ml of BHT the corresponding values were 82.24, 81.76, and 73.03% of inhibition, respectively. Conclusion: The study results showed that gossypin has significant antioxidant activity.