Journal
TREE PHYSIOLOGY
Volume 27, Issue 12, Pages 1721-1730Publisher
OXFORD UNIV PRESS
DOI: 10.1093/treephys/27.12.1721
Keywords
caulogenesis; conifers; cytokinins; organogenesis; SSH; stone pine
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As part of a study aimed at understanding the physiological and molecular mechanisms involved in adventitious shoot bud formation in pine cotyledons, we conducted a transcriptome analysis to identify early-induced genes during the first phases of adventitious caulogenesis in Pinus pinea L. cotyledons cultured in the presence of benzyladenine. A subtractive cDNA library with more than 700 clones was constructed. Of these clones, 393 were sequenced, analyzed and grouped according to their putative function. Quantitative real-time PCR analysis was performed to confirm the differential expression of 30 candidate genes. Results are contrasted with available data for other species.
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