Plasma membrane ultrastructure in embryogenic cultures of orchardgrass during NaCl stress

Embryogenic cultures of Dactylis glomerata L. were subjected to NaCl stress by culturing for 4 passages in Schenk and Hildebrandt (SH) medium containing 30 mu M dicamba and 200 mM NaCl. Ultrastructural studies indicated invaginations and disruptions of the plasma membrane. Membrane-bound vesicles were observed in the cytoplasm of NaCl treated cells and their occurrence were increased with the culture age.