Journal
MEMORIAS DO INSTITUTO OSWALDO CRUZ
Volume 102, Issue 8, Pages 919-924Publisher
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762007000800005
Keywords
Aedes aegypti; gut diverticulum; microbiota; Serratia sp.
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We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
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