Journal
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Volume 82, Issue 5, Pages 846-854Publisher
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2010.09-0399
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Funding
- FIC NIH HHS [D43 TW007129-08, D43 TW007129] Funding Source: Medline
- NHLBI NIH HHS [R01 HL073732, HL-73732, R01 HL073732-08, R01 HL073732-07] Funding Source: Medline
- NIAID NIH HHS [AI-076248, R01 AI076248] Funding Source: Medline
- PHS HHS [D43 W007129] Funding Source: Medline
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We examined the extent to which different Thpanosoma cruzi strains induce transcriptomic changes in cultured L6E9 myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (IC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.
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