Journal
JOURNAL OF BACTERIOLOGY
Volume 189, Issue 24, Pages 8786-8792Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00977-07
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Funding
- Biotechnology and Biological Sciences Research Council [BB/C514266/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/C514266/1] Funding Source: Medline
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The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in Delta fnr, Delta crp, and Delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that nonmotile progeny are found in some MG1655 directed deletion mutants, and studies on the effects of gene knockouts should be viewed with caution when the mutants have not been screened for the presence of secondary deletions or confirmed by other methods.
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