Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 13, Issue 12, Pages 1199-1201Publisher
ELSEVIER SCI LTD
DOI: 10.1111/j.1469-0691.2007.01819.x
Keywords
Acinetobacter; genomic species; gyrB genes; identification; PCR; speciation
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A new PCR-based method that exploits differences in gyrB gene sequences was developed to distinguish between Acinetobacter baumannii and Acinetobacter genomic sp. 13TU. Among 118 clinical and reference Acinetobacter strains, 102 of which were previously speciated by amplified rDNA restriction analysis as belonging to the Acinetobacter calcoaceticus-A. baumannii complex, the method correctly identified 31 A. baumannii and 54 Acinetobacter genomic sp. 13TU isolates to the species level. The method was rapid, specific and easy to interpret.
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