Journal
JOURNAL OF SEPARATION SCIENCE
Volume 30, Issue 18, Pages 3207-3213Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200700262
Keywords
flavonoids; Herba Epimedii; HPLC-UV; simultaneous determination; qualitative evaluation
Categories
Ask authors/readers for more resources
A simple high performance liquid chromatographic method has been developed for the determination of nine flavonoids in Herba Epimedii, including quercetin-3-O-glucoside, epimedin A, hexandraside A, epimedin B, epimedin C, icariin, icariside 1, icariside 11, and icaritin. The chromatographic separation was performed on a Diamonsil C-18 column (5 mu m, 250 x 4.6 mm) with gradient elution by acetonitrile-0.5% acetic buffer (adjusted to pH 5.00 with triethylamine). Methodological validation gave acceptable linearities (r(2) >0.9992) and recoveries (ranging from 98.9 to 102.4%). The limits of detection of these flavonoids ranged from 16.3 to 83.3 ng. The results indicated that the contents of flavonoids in Herba Epimedii varied significantly from habitat to habitat with contents ranging from 0.05 to 39.0 mg/g. Twenty five Herba Epimedii samples prepared from five different botanical materials were investigated by the established method, and the results showed the influence of the botanical material and the habitat on the quality of Herba Epimedii. The proposed method is simple, effective, and suitable for the evaluation of this traditional Chinese medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available