Journal
NEUROCHEMICAL RESEARCH
Volume 32, Issue 12, Pages 2015-2022Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-007-9340-y
Keywords
human fetal tissue; neurons; oligodendroglia; microglia; astroglia; neurodegeneration
Categories
Funding
- NINDS NIH HHS [R21 NS048923, R21 NS048923-03, NS48923, R01 NS039940-07, R01 NS039940, NS39940] Funding Source: Medline
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Elucidation of the underlying pathogenic mechanisms leading to apoptosis of neurons and oligodendrocytes and activation of microglia and astrocytes in different neurodegenerative and neuroinflammatory disorders remains a challenge in neuroscience. In order to overcome the challenge and find out therapeutic remedies, it is important to study live and death processes in each and every cell type of the brain. Here we present a protocol of isolating highly purified microglia, astrocytes, oligodendrocytes, and neurons, all four major cell types of the CNS, from the same human fetal brain tissue. As found in vivo, these primary neurons and oligodendroglia underwent apoptosis and cell death in response to neurodegenerative challenges. On the other hand, astroglia, and microglia, cells that do not die in neurodegenerative brains, became activated after inflammatory challenge. The availability of highly purified human brain cells will increase the possibility of developing therapies for different neurodegenerative disorders.
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