Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 12, Pages 2007-2012Publisher
SPRINGER
DOI: 10.1007/s10529-007-9438-y
Keywords
biological activity; human epidermal growth factor; matrix attachment regions; optimized codons; tobacco
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To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.
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