Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 328, Issue 1-2, Pages 97-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2007.08.011
Keywords
L-selectin; neutrophil; shear; receptor; clustering
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Funding
- NHLBI NIH HHS [HL018208, P01 HL018208, P01 HL018208-32] Funding Source: Medline
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L-selectin on leukocytes is critical in leukocyte tethering and adhesion to inflamed endothelium and lymphocyte homing to lymphoid organs. The spatial distribution of L-selectin on leukocytes controls cellular adhesive function in hydrodynamic shear How L-selectin changes its position on the cell membrane remains an open question, but a possible candidate is shear stress encountered on the cell surface. Here we demonstrate shear-induced L-selectin polarization on the membrane during the process of centrifugation of resting neutrophils Via immunofluorescent microscopy. It was found that randomly distributed L-selectin on neutrophils moves to a polar cap at one end of the cell after centrifugation (300 xg for 2 min) without inflammatory stimuli. This L-selectin redistribution under shear was predicted by Monte Carlo simulations that show how convection dominates over diffusion, leading to L-selectin cap formation during centrifugation at 280 xg or during leukocyte adhesion to the endothelial wall at 1 dyn/cm(2). Those results point to a role for shear stress ir. the modulation of L-Selectin distribution, and suggest a possible alternate mechanism and reinterpretation of previous in vitro studies of L-selectin mediated adhesion of neutrophils isolated via centrifugation. (C) 2007 Elsevier B.V. All rights reserved.
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