Journal
AMERICAN JOURNAL OF TRANSPLANTATION
Volume 13, Issue 12, Pages 3253-3261Publisher
WILEY
DOI: 10.1111/ajt.12487
Keywords
Extended donor criteria; genotyping; infectious diseases; PCR; transplantation
Categories
Funding
- National Scientific and Technical Research Council (CONICET) [PIP 112-200801-02915]
- National Agency of Science and Technology [PICT 33955/2005, PICT 0207/2011]
- SANOFI-AVENTIS
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Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naive TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36-98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment. Polymerase chain reaction strategies allow for the early diagnosis of acute Chagas disease in naive recipients transplanted with organs from Trypanosoma cruzi-infected donors. See editorial by Machado on page 3065.
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