Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 41, Issue 5, Pages 548-557Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2007.07.006
Keywords
hemoglobin; DTAB; SDS; hemichrome; isoelectric point
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The interactions of hemoglobin (Hb) with sodium dodecyl sulfate (SDS) and dodecyl trimethylammonium bromide (DTAB) are investigated by several methods. We observed the formation of hemichrome below the critical micelle concentration (cmc) of surfactant and the release of heme from Hb above the cmc. When pH value of Hb/surfactant system is lower than isoelectric point (pI) of Hb, the interaction of SDS with Hb is both electrostatic and hydrophobic, while the interaction of DTAB with Hb is hydrophobic mainly. On the contrary, when pH >pI, the interaction of SIDS with Hb is hydrophobic mainly, while the interaction of DTAB with Hb is both electrostatic and hydrophobic. In the case where both the electrostatic interaction and hydrophobic interaction exist, the electrostatic interaction plays a more. important role. Thus, SDS tends to interact with Hb more obviously than DTAB does when pHpI. (C) 2007 Published by Elsevier B.V.
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