Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 34, Issue 12, Pages 807-811Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-007-0257-x
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In this study, we developed a fluorescence-based assay for quantifying the aromatic amino acid L-tyrosine in small sample volumes employing 96-well microtiter plates. The method was based on the specific derivatisation of L-tyrosine with 1-nitroso-2-naphthol and the formation of a stable complex for the fluorescent determination of L-tyrosine concentrations. The original procedure for L-tyrosine measurements in blood or tissue samples (Waalkes and Udenfriend in J Lab Clin Med 50: 733 - 736, 1957) was modifed to a simple assay suitable for high-throughput screening of L-tyrosine producing microorganisms such as engineered Escherichia coli.
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