Journal
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
Volume 32, Issue 6, Pages 1148-1155Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/H07-131
Keywords
urate; ferric reducing ability of plasma; F2-isoprostanes; probenecid; oxidative stress; exercise
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Funding
- NCI NIH HHS [CA-77839] Funding Source: Medline
- NCRR NIH HHS [RR00095] Funding Source: Medline
- NIDDK NIH HHS [DK-48831] Funding Source: Medline
- NIGMS NIH HHS [GM-15431] Funding Source: Medline
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Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at similar to 80% VO2 (max) under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a double-blind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) x 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey-Kramer multiple comparison tests, and Student's t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p <= 0.05), and group effects existed for the exercise trials (p = 0.023 and p <= 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.
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